The following modules provide fundamental information that you will need to know in order to perform testing in the Immunohematology Reference Lab (IRL). You’ll want to follow along with your handbook while you work through the modules, as your handbook contains valuable supplemental information and provides a place to take notes.
(Modules are best viewed using Chrome as a browser.)
This module provides a review of pre-transfusion testing and explains the antibody identification process, including interpreting antigrams and performing rule outs. It is advisable to print out the document entitled “Module 3 Panels,” so that you can follow along.
This module discusses the importance of antigen typing both patients and donors. The difference between a serologic phenotype and a molecular genotype is discussed. Finally, calculations to determine the probability of finding antigen-negative units are reviewed.
This module describes the process of adsorbing warm autoantibody in cases of panreactivity, in order to identify underlying alloantibodies. You may want to print out the handout “Module 7 Practice Rule-outs with Differential Adsorptions” so that you can follow along.
This module describes the use of inhibition (neutralization) of antibodies during antibody identification. It covers what inhibition is and when it is used. In addition, it includes case studies that demonstrate how to interpret the results of inhibition studies.